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The maintenance of electron transfer and oxygen evolution activity
Thus, although the AC mutant can survive a prolonged incubation normal growth at such temperature requires supplement. Notably, the growth of wild type Synechocystis showed similar kinetics to that of DKS with somewhat higher growth during the first three days of incubation followed by a slower decay from day 4. However, VE-821 ATM/ATR inhibitor when grown the Chl content sharply declined in DKS after 3 days, whereas that in the double mutant increased throughout the entire period of incubation to almost 3 times its initial value. Thus, on the fifth day of incubation the D1 content dropped to,4% of its initial value and that of Rubisco reached non-significant levels for DKS. To decipher the predominant contribution, we monitored the D1 content as Staurosporine PKC inhibitor a function of time in cells exposed to high light irradiances, either in the presence or in the absence of the protein synthesis inhibitor lincomycin. The D1 protein content in both the AC and DKS decayed during incubation at 43uC and under high light conditions. However, the decay of the D1 protein content in the mutant was markedly slower, reaching 20% of its initial value after 6 h of illumination. Lincomycin markedly accelerated the decay of D1 content in both DKS and AC cultures. Nevertheless, after 6 hours the D1 protein content in the AC reached,5–10% of the initial value whereas in DKS it dropped to zero.