Saab NewsSaab ForumSaab ClassifiedsSaab SpecificationsAbout UsSaab links

Saabing.com

Saab Discussion Boards
It is currently Sun Jun 17, 2018 10:43 pm

All times are UTC - 5 hours




Post new topic Reply to topic  [ 1 post ] 
Author Message
 Post subject: In view of the aforementioned observations and consideration
PostPosted: Tue Jan 30, 2018 12:25 am 
Offline
Saab Junkie

Joined: Wed Jul 31, 2013 1:53 am
Posts: 205
In view of the aforementioned observations and considerations we hypothesized
Concomitant D1-Ser209Ala and D1- Ser212Cys mutations may improve the functional stability of PSIIRC in mesophilic cyanobacteria grown at elevated temperatures. We further hypothesized that enhanced concentration should compensate for the increased Rubisco affinity to oxygen at elevated temperatures and thereby reduce the impairment of the D1 repair mechanism. PCC 6803 which was selected as control because it retains only the intact wild-type psbAII gene followed by BI-D1870 S6 Kinase inhibitor a kanamycin resistance gene. The absence of the other two gene copies, psbAI and III genes, which are replaced by spectinomycin and chloramphenicol resistance cartridges, simplifies the interpretation of genetic modifications in the D1 protein subunit. There was no significant difference in the growth rates and pigmentation between the two strains when grown at 30uC under normal air bubbling. When incubated at the growth of mutant was slightly slower, in comparison to the control strain that showed a much slower growth at both temperatures. Importantly, when grown at the same temperature but under stirring the AC Oligomycin A ATPase inhibitor biomass increased at relatively slow pace and started to level off at the fourth day. Nevertheless, when transferred back to 30uC the growth was regained. In contrast, under the same conditions, the DKS cultures leveled off after three days of slow growth and could not recover when transferred back to 30uC.


Top
 Profile E-mail  
 
Display posts from previous:  Sort by  
Post new topic Reply to topic  [ 1 post ] 

All times are UTC - 5 hours


Who is online

Users browsing this forum: No registered users and 2 guests


You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
cron
Powered by phpBB © 2000, 2002, 2005, 2007 phpBB Group